The Bradford Reagent is used for rapid and economical estimation of protein concentration. It works on the principle that Coomassie Brilliant Blue G-250 dye binds to proteins, resulting in a color change that can be measured spectrophotometrically. Upon binding, the dye–protein complex shows a shift in absorbance, allowing accurate quantification of protein levels. The assay is simple, fast, and suitable for routine protein analysis in research laboratories.

• Product: Bradford Reagent

• Assay type: Colorimetric protein estimation assay

• Principle: Based on binding of Coomassie Brilliant Blue G-250 (CBBG) dye to proteins, causing a shift in absorbance from 465 nm to 595 nm

• Measurement wavelength: 595 nm

• Protein standard: Bovine Serum Albumin (BSA) commonly used

• Sensitivity: More sensitive to BSA than many other proteins

• Detection range: Approximately 20–1500 µg/mL protein

• Performance:

  • Provides accurate determination of protein concentration

  • Simple, rapid, and easy to use

  • Does not require preparation of working standards

  • Faster and easier than traditional Lowry method

• Research use: For research use only

• Storage condition: Store at 2–8 °C

• Expiry: Refer to package for details

• Quantitative estimation of protein concentration in biological samples