Edna MaximaFast cDNA Synthesis Kit is a complete system for efficient first-strand cDNA synthesis from mRNA or total RNA. It includes dsDNase, which selectively degrades double-stranded DNA without affecting single-stranded DNA, ensuring RNA templates are free from contaminating genomic DNA. This is especially useful when RNA is extracted using phenol-based or column-based methods. The kit also contains M-MuLV Enzyme Mix (with M-MuLV Reverse Transcriptase and Murine RNase Inhibitor) and M-MuLV Reaction Mix (with dNTPs, buffer, oligo(dT), and random hexamer primers), optimized for targets under 1 kb. It delivers excellent results in both conventional and real-time RT-PCR.

• Contamination Control: dsDNase degrades only double‑stranded DNA; single‑stranded DNA is unaffected
• Performance: Optimized for targets <1 kb; suitable for conventional and real‑time RT‑PCR
• Enzyme System: M‑MuLV Reverse Transcriptase with Murine RNase Inhibitor (in Enzyme Mix)
• Primer System: Optimized blend of oligo(dT) and random hexamer primers (in Reaction Mix)
• Buffer Chemistry (1X): Tris‑HCl, KCl, MgCl₂, each dNTP at 0.5 mM, DTT at 10 mM; pH 8.3 ± 0.25 at 25°C
• Inhibitors of Activity: Metal chelators, inorganic phosphate, pyrophosphate, polyamines
• Storage: Store at –20°C
  Spin the tubes briefly before use
• Heat Inactivation: 95°C for 1 minute

• First strand cDNA synthesis for RT-PCR and real-time RT-PCR
• Synthesis of cDNA for cloning and expression.
• Generation of labelled cDNA probes for microarrays.
• DNA labelling
• Analysis of RNA by primer extension