The Bradford Protein Assay is a fast, cost-effective method to measure protein concentration. It uses Coomassie Brilliant Blue G-250 (CBBG) dye, which binds to proteins and shifts absorbance from 465 nm to 595 nm. By measuring absorbance of known BSA standards, a regression equation is generated to estimate unknown protein concentrations. BSA is preferred due to its high sensitivity in this assay.

• Storage Temperature: 2–8°C
• Contents: Bradford Reagent (500 mL), BSA Protein Standard (1 mg/mL, 5 mL)
• Assay Sensitivity Range: 20 µg to 1500 µg/mL
• Assay Capacity: At least 160 standard 3 mL assays
• Reagent Type: Ready-to-use, no need to prepare working standards Measurement
• Wavelength: 595 nm (absorbance shift from 465 nm to 595 nm upon protein binding)
• Preferred Standard Protein: Bovine Serum Albumin (BSA) Assay
• Principle: Colorimetric detection using Coomassie Brilliant Blue G-250 dye
• Protein-Dye Interaction: Binding via positively charged and hydrophobic residues

• Accurate protein quantification across various samples

• Visual or spectrophotometric estimation at 595 nm

• Ready-to-use reagent; no need for working standard prep

• Faster and simpler than the Lowry method

• Detects protein concentrations from 20 µg to 1500 µg/mL

• Supports at least 160 standard 3 mL assays