• Concentration: Protein concentration is determined by measuring the absorbance at 280 nm.
• Purity: 12% reducing SDS PAGE confirms ≥98% purity.
• Specific activity ≥ 1 U/μL protein.
• Unit Definition: 1 unit of TEV Protease will cleave 2 μg of MBP-fusion protein, to 95% completion in a total reaction volume of 10 μl in 1 hour at 30°C in 50 mM Tris-HCl (pH 8 @ 25°C) with 0.5 mM EDTA and 1 mM DTT.
• Store at –20°C
  Mix the tube gently before use

 Removal of affinity purification tags
with TEV site present in the sequence

• • Recombinant Enterokinase Light Chain

TEV Protease is a highly specific cysteine protease. The TEV Protease recognition sequence with the highest catalytic efficiency is ENLYFQ▼S; however, the amino acid in the P1' position can also be G, A, M, C, or H. It is often used for the removal of affinity purification tags such as maltose-binding protein (MBP) or poly- histidine from fusion proteins. TEV Protease has a 7xHis-tag for easy removal from a reaction using nickel affinity resins.