HotStart Taq PCR Master Mix (2X) is a ready-to-use, premixed solution containing a chemically modified Taq DNA polymerase, dNTPs, MgCl₂, and reaction buffer at optimal concentrations for efficient amplification of DNA templates by PCR. The enzyme remains inactive at room temperature, preventing non-specific amplification and primer-dimer formation during reaction setup. Upon heating to 95 °C during the initial denaturation step, the enzyme is reactivated and supports specific DNA amplification. The master mix minimizes pipetting errors, miscalculations, and contamination, and is compatible with general PCR-based application protocols.

Source: An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus.

• HotStart Taq PCR Master Mix (2X) is a ready-to-use, premixed solution containing chemically modified Taq DNA polymerase, dNTPs, MgCl₂, and reaction buffer at optimal concentrations

• HotStart Taq DNA polymerase is a chemically modified thermostable enzyme

• Enzyme activity is inactive at room temperature, preventing non-specific amplification and primer-dimer formation during reaction setup

• Enzyme becomes reactivated upon heating to 95 °C, restoring full polymerase activity without affecting subsequent PCR reactions

• Possesses 5’→3’ polymerase activity

• Compatible with general PCR-based application protocols

• Enables amplification of DNA fragments up to 5 kb under routine PCR conditions

• Purity confirmed to be ≥98% by 10% reducing SDS-PAGE

• Performance validated through PCR-based activity assays

• No contaminating endonuclease or exonuclease activity detected

• Shipped on blue ice

• Store at –20 °C upon arrival

• HotStart Taq PCR Master Mix (2X) is stable at 4 °C for three months or up to fifteen freeze–thaw cycles

• For routine daily use, storage at 4 °C is recommended

• Spin tubes briefly before use to collect contents at the bottom

•  PCR
•  Primer Extension
•  Colony PCR
•  Other allied applications

• High-fidelity Pfu PCR Master Mix (2X)
• CrimRed Taq PCR Master Mix (2X)
• Taq PCR Master Mix (2X)
• CrimRed Taq PCR Master Mix, Lyophilized (2X)
• CrimRed Hotstart Taq PCR Master Mix (2X)
• CrimRed High-fidelity Pfu PCR Master Mix (2X)

Technical Data Sheet