Taq DNA polymerase has a molecular weight of 94 kDa and is used for routine PCR amplification reactions. It possesses 5’à3’ polymerase activity. It is supplied with 10X Standard reaction buffer (with MgCl2). The 10X reaction buffer with MgCl2 is compatible with general PCR based application protocols.

Source: An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus.

• Taq DNA Polymerase exhibits 5’→3’ polymerase activity and is suitable for routine PCR amplification

• Enzyme is derived from Thermus aquaticus and expressed in E. coli

• Molecular weight is approximately 94 kDa

• Supplied glycerol-free

• Compatible with general PCR-based application protocols

• Provided with 10X standard reaction buffer containing MgCl₂

• Capable of amplifying DNA fragments up to 5 kb under routine conditions

• Purity confirmed to be ≥98% by 10% reducing SDS-PAGE

• Enzyme activity validated through PCR-based activity assays

• RNase activity: No degradation of RNA detected after incubation

• Non-specific DNase activity: No detectable nuclease degradation observed

• Storage: Store at 4 °C 

• Spin tubes briefly before use to collect contents at the bottom

• Refer to package for expiry details

•  PCR
•  Primer Extension
•  Colony PCR
•  Other allied applications

• High-fidelity Pfu PCR Master Mix (2X)
• Hotstart Taq PCR Master Mix (2X)
• High-fidelity Pfu DNA Polymerase 5 U/μL with Mg-free Buffer
• High-fidelity Pfu DNA Polymerase 5 U/μL with 5X GC-Rich Buffer
• Taq DNA Polymerase

Technical Data Sheet