High-fidelity PCR Master Mix (2X) consists of two components: Edna Pfu PCR Master Mix Buffer and Edna Pfu DNA Polymerase. The premixed buffer contains dNTPs, MgSO₄, and optimized reaction components to enable efficient DNA amplification by PCR. The Pfu DNA polymerase, isolated from Pyrococcus furiosus and expressed in E. coli, possesses 3’→5’ proofreading activity, ensuring accurate nucleotide incorporation. The two components are supplied separately to enhance stability and are mixed to obtain a 2X master mix. This formulation reduces pipetting errors, miscalculations, and contamination, enabling highly reproducible PCR results. The master mix is compatible with general PCR-based applications.

Source: An E. coli strain that carries the Pfu DNA Polymerase gene

• Contains Pfu DNA polymerase with high-fidelity 3’→5’ exonuclease proofreading activity

• Molecular weight of Pfu DNA polymerase is approximately 90 kDa

• Provides accurate and reliable DNA amplification

• Suitable for amplification of DNA fragments up to 5 kb under routine conditions

• Tested for purity (≥98% by reducing SDS-PAGE)

• Free from contaminating nuclease activity

• Enzyme activity confirmed through PCR-based activity assays

• Shipped on blue ice

• Storage: Store at –20 °C upon receipt

• Pfu Polymerase 2X Master Mix is stable at 4 °C for three months or up to fifteen freeze–thaw cycles

• For routine daily use, storage at 4 °C is recommended

• Spin tubes briefly before use to collect contents at the bottom

• High-fidelity PCR
• cDNA cloning and expression
• Mutation analysis
• Other allied applications

• CrimRed Taq PCR Master Mix (2X)
• Hotstart Taq PCR Master Mix (2X)
• High-fidelity Pfu DNA Polymerase 5 U/μL with Mg-free Buffer
• High-fidelity Pfu DNA Polymerase 5 U/μL with 5X GC-Rich Buffer
• Taq DNA Polymerase

Technical Data Sheet