Taq DNA Polymerase is a thermostable DNA polymerase with 5´→ 3´ polymerase activity and 5´ flap endonuclease activity. Edna Taq products are recombinantly produced in E. coli, are thus highly pure, and offer robust and reliable PCR activity.

What's In The Box:

The product includes a vial of the formulated recombinant enzyme along with 10X Taq reaction buffer which is detergent-free and designed to be compatible with existing assay systems.Highlights:

• Isolated from a recombinant source
• The enzyme is free of any tags
• Supplied with 10X Reaction Buffer
• Robust and reliable reactions
• Tolerates a wide range of templates
• Incorporates dUTP, dITP and fluorescently-labeled nucleotides
• An ideal tool for amplifying DNA of length <5 kb

Product Source

An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus

Unit Definition: One unit is defined as the amount of enzyme that will incorporate 15 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C. 

Storage Temperature: -20 C

Molecular Weight: 94 kDa

Activity: 5'-3' exonuclease (not 3'-5') resulting in single-base 3' overhangs

Heat Inactivation: None10x Taq Reaction Buffer:

• 100 mM Tris-HCl
• 500 mM KCl
• 15 mM MgCl2

(pH 8.3)

Edna Taq DNA Polymerase products are well suited to a range of molecular biology applications:

• Polymerase Chain Reaction (PCR)
• Primer Extension
• Colony PCR
• Microarray Analysis
• DHPLC

• High-fidelity Pfu PCR Master Mix (2X)
• CrimRed Taq PCR Master Mix (2X)
• Hotstart Taq PCR Master Mix (2X)
• High-fidelity Pfu DNA Polymerase 5 U/μL with Mg-free Buffer | EDNA
• High-fidelity Pfu DNA Polymerase 5 U/μL with 5X GC-Rich Buffer | EDNA

Technical Data Sheet