Taq DNA Polymerase is a thermostable DNA polymerase with 5´→ 3´ polymerase activity and 5´ flap endonuclease activity. Edna Taq products are recombinantly produced in E. coli, are thus highly pure, and offer robust and reliable PCR activity.

What's In The Box:

The product includes a vial of the formulated recombinant enzyme along with 10X Taq reaction buffer which is detergent-free and designed to be compatible with existing assay systems.Highlights:

• Isolated from a recombinant source
• The enzyme is free of any tags
• Supplied with 10X Reaction Buffer
• Robust and reliable reactions
• Tolerates a wide range of templates
• Incorporates dUTP, dITP and fluorescently-labeled nucleotides
• An ideal tool for amplifying DNA of length <5 kb

Product SourceAn E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus

Unit Definition: One unit is defined as the amount of enzyme that will incorporate 15 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C. Storage Temperature: -20 CMolecular Weight: 94 kDaActivity: 5'-3' exonuclease (not 3'-5') resulting in single-base 3' overhangsHeat Inactivation: None10x Taq Reaction Buffer:

• 100 mM Tris-HCl
• 500 mM KCl
• 15 mM MgCl2

(pH 8.3)

Edna Taq DNA Polymerase products are well suited to a range of molecular biology applications:

• Polymerase Chain Reaction (PCR)
• Primer Extension
• Colony PCR
• Microarray Analysis
• DHPLC

• Taq DNA Polymerase with 10x Buffer (Mg-free) and MgCl2
• High-fidelity Pfu DNA Polymerase with 10x Buffer
• High-fidelity Pfu DNA Polymerase with 10x Buffer (Mg-free) and MgCl2
• T4 DNA Ligase Standard Concentration with 10x Ligation Buffer
• T4 DNA Ligase High Concentration with 10x Ligation Buffer
• T4 DNA Ligase Rapid/Blunt-End Ligation Kit
• Ultra-RNase A, Recombinant
• EdnaBeads Plasmid DNA Extraction Kit (Coming Soon!)
• EdnaBeads DNA Clean-Up Kit (Coming Soon!)

Extra Buffers and Reagents for Molecular Biology (for Taq DNA Polymerase, Pfu DNA Polymerase, RNase A, and T4 DNA Ligase reactions)