Pfu DNA Polymerase is a thermostable DNA polymerase that has 5´→ 3´ polymerase activity and 5´ flap endonuclease activity. The enzyme also exhibits 3´→5´ exonuclease (proofreading) activity.  Base misinsertions are rapidly removed by the polymerase, making it a good option for applications where high-fidelity error-free synthesis is required.  Edna Pfu products are recombinantly produced in E. coli and thus are highly pure and offer robust and reliable PCR activity.

What's In The Box:

The product includes a vial of the formulated recombinant enzyme along with 10X Pfu reaction buffer which is detergent-free and designed to be compatible with existing assay systems. 

Highlights:

• Isolated from a recombinant source

• The enzyme is free of any tags

• Supplied with 10X Reaction Buffer

• Robust and reliable reactions

• Tolerates a wide range of templates

• Incorporates dUTP, dITP and fluorescently-labeled nucleotides

• An ideal tool for amplifying DNA of length <5 kb

Product Source

An E. coli strain that carries the Pfu DNA Polymerase gene from Pyrococcus furiosus.

Unit Definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nanomoles of dNTPs into acid insoluble material in 30 minutes at 75°C. 

Storage Temperature: -20 C

Molecular Weight: 92 kDa

Activity: 5'-3' exonuclease (not 3'-5') resulting in single-base 3' overhangs. The enzyme also exhibits 3´→ 5´ exonuclease (proofreading) activity. 

Heat Inactivation: None

10x Pfu Reaction Buffer:

• 200 mM Tris-HCl

• 100 mM KCl

• 100 mM (NH₄)₂SO₄ 

• 10 mM MgSO4

• 1% Triton X-100

(pH 8.8)

Edna Pfu DNA Polymerase products are well suited to a range of molecular biology applications:

• Polymerase Chain Reaction (PCR)

• Primer Extension

• Colony PCR

• Microarray Analysis

• DHPLC

• High-fidelity Pfu PCR Master Mix (2X)
• CrimRed Taq PCR Master Mix (2X)
• Hotstart Taq PCR Master Mix (2X)
• High-fidelity Pfu DNA Polymerase 5 U/μL with 5X GC-Rich Buffer
• Taq DNA Polymerase

Technical Data Sheet