EdnaQuickPNGase F Reaction Mix
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Product Description
Edna Quick PNGase F combines high activity PNGase F with an improved reaction buffer which facilitates quick deglycosylation of antibodies and fusion proteins in mere minutes instead of > 4
hours. The N-Glycosidase PNGase F is recombinant enzyme cloned from Elizabethkingia miricola. It has molecular
weight of ~36 Kda. PNGase F catalyzes the cleavage of N-linked oligosaccharides between the innermost GlcNAc and
asparagine residues of high mannose, hybrid and complex oligosaccharides from N-linked glycoproteins. PNGase F will not
remove oligosaccharides containing Alpha- (1,3)-linked core fucose commonly found on plant glycoproteins.
Source: An E. coli strain that carries the gene which express PNGase F.
Properties and Storage
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Product: EdnaQuick PNGase F
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Type: Recombinant N-Glycosidase F (rDNA product)
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Source: E. coli strain expressing PNGase F from Elizabethkingia miricola
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Molecular weight: ~36 kDa
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Function: Rapid cleavage of N-linked oligosaccharides between the innermost GlcNAc and asparagine residues of high-mannose, hybrid, and complex glycoproteins
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Specificity: Does not remove oligosaccharides containing α(1,3)-linked core fucose (common in plant glycoproteins)
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Performance: Enables rapid deglycosylation of antibodies and fusion proteins within minutes
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Unit definition: One unit removes >95% of carbohydrate from 10 µg of denatured RNase B in 1 hour at 37 °C in a 10 µL reaction
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Purity: ≥98% purity confirmed by 10% reducing SDS-PAGE
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Protein concentration: Determined by UV absorbance at 280 nm
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Research use: For research use only
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Enzyme storage: Store at 2–8 °C
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Reaction buffer storage: Store at –20 °C
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Handling notes: Spin tubes briefly before use
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Expiry: Refer to package for details
Application
- Characterization of glycoprotein
- Determining location of glycosylation on the protein
- Glycan structure determination
- Monoclonal antibody characterization