Glu-C, Sequencing Grade, is a serine protease that specifically cleaves peptide bonds at the C-termini of glutamic acid
residues. It also cleaves at aspartic acid residues at a 100-300 times slower rate.

Source: An E. coli strain that carries the gene which expresses Endoproteinase GluC from Staphylococcus aureus V8.
Added to mass spectrometry digestion

• Function: Cleaves peptide bonds at the C-terminal of glutamic acid; slower cleavage at aspartic acid

• Delivery Form: Lyophilized with trehalose

• Preferred Usage Concentration: 100 ng/μL

• Activity Range: Optimal between pH 4–9

• Buffer Compatibility:

  • Specificity for glutamic acid in ammonium bicarbonate/acetate

  • Cleaves both Glu and Asp in phosphate buffers

  • Active in up to 20% acetonitrile, 0.2% SDS, 0.8M urea, 0.2M guanidine HCl

• Purity: ≥95% confirmed by SDS-PAGE

• Activity Assay: Complete digestion of beta insulin at 25°C for 1 hour verified by HPLC

• Lyophilized Enzyme: Store at –20°C

• Reconstituted Enzyme: Store at –70°C for up to 8 weeks

The enzyme is suitable for:

•  Peptide mapping
• Fingerprinting
• Sequencing analysis

• IdeS Protease, Lyophilized, Mass Spectrometry Grade, 50 U/μL
• Recombinant Carboxypeptidase B (Frozen, Glycerol-Free)
• Antibody Standard for PNGase F
• EdnaQuickPNGase F Reaction Mix
• PNGase F (Glycerol and EDTA Free), 500 U/μL
• PNGase F, Glycerol-free, 500 U/μL
• PNGase F, 500 U/μL
• Modified Trypsin, Lyophilized

Technical Data Sheet