Edna Carboxypeptidase B (CPB) is a 35.1kDa protein consisting of 307 amino acids. It sequentially cleaves C-terminal Lysine,
arginine and Histidine residues. It shows activity in the pH range of 5-12 with maximum activity at pH 8. CPB has pI value of ~6. CPB activity depends on ZN+2 ions. CPB activity increases with the increase in temperature.

Source: An E. coli strain which expresses Rat Carboxypeptidase B.

• Product: Recombinant Carboxypeptidase B (Lyophilized)

• Type: Recombinant (rDNA product)

• Source: E. coli strain expressing rat Carboxypeptidase B

• Molecular weight: ~35.1 kDa protein (~307 amino acids)

• Enzyme activity: Cleaves C-terminal basic amino acids (lysine, arginine, histidine)

• Optimal pH: 5–12, with maximum activity at pH ~8

• Cofactor requirement: Activity dependent on Zn²⁺ ions

• Temperature effect: Enzyme activity increases with increase in temperature

• Unit definition: One unit hydrolyzes 1 µmol of Hippuryl-L-Arg per minute at 30 °C and pH 9.5

• Purity: ≥98% purity confirmed by 10% reducing SDS-PAGE

• Protein concentration: Determined by UV absorbance at 280 nm

• Research use: For research use only

• Storage temperature: Store at –20 °C

• Form: Supplied in lyophilized form

•  Characterization of Monoclonal antibodies (mAb)
•  Removing of N terminal basic amino acids
•  Manufacturing of Insulin and Insulin derivatives
•  Protein peptide mapping

• IdeS Protease, Lyophilized, Mass Spectrometry Grade, 50 U/μL
• Glu-C Sequencing Grade
• Antibody Standard for PNGase F
• EdnaQuickPNGase F Reaction Mix
• PNGase F (Glycerol and EDTA Free), 500 U/μL
• PNGase F, Glycerol-free, 500 U/μL
• PNGase F, 500 U/μL
• Modified Trypsin, Lyophilized

Technical Data Sheet