Mass Spectrometry


Product Categories

Enzymes

Trypsin (Sequencing Grade)

Trypsin is a serine protease from bovine pancreas that cleaves at the carboxylic side of lysine and arginine residues. Edna trypsin is highly specific as it is purified by ion exchange chromatography, modified by reductive methylation to prevent auto-cleavage, and TPCK-treated to inactivate any remaining chymotrypsin activity.

PNGase F (Recombinant)

PNGase F is a glycosidase cloned from Elizabethkingia miricola. It cleaves N-linked oligosaccharides and is used to study protein glycosylation. Digestion with PNGase F is the most effective way to remove N-linked glycans. Use our Quick Action reaction mix and custom protocol for 10 minute reactions (instead of >4 hours!)

Chymotrypsin (Sequencing Grade)

Chymotrypsin is a serine protease from bovine pancreas that cleaves at the carboxylic side of aromatic tyrosine, phenylalanine and tryptophan residues. It is highly purified by ion exchange chromatography for best specificity for sequencing. It is also well-suited for in-process applications such as multimer cleavage.

Carboxypeptidase B (Recombinant)

Carboxypeptidase B (CPB) is a pancreatic endo-peptidase that cleaves C-terminal basic residues, particularly Arg and Lys. Edna CPB products are well-suited for characterization applications such as analysis of mAb basic variants, and for process applications such as insulin cleavage or reduction of heterogeneity in mAb material.

Products:
Carboxypeptidase B, Lyophilized

Antibody Standard for Carboxypeptidase B

Lysine C (Recombinant)

Coming Soon!

Glu C (Recombinant)

Coming Soon!

IdeS Protease (Recombinant)

Coming Soon!

Endoglycosidase S (Recombinant)

Coming Soon!

Pepsin (Extrapure)

Coming Soon!

Asp N (Recombinant)

Coming Soon!

Reagents and Kits

EdnaPRO Digestion Improver

EdnaPRO Digestion Improver is a mass spectrometry compatible surfactant which improves the performance of proteases including Trypsin, Chymotrypsin and Lys-C for both in-gel and solution digestions, enhancing the protease action at diffcult-to-access sites and improving the recovery of longer peptides from gels.

Antibody Standard

Edna's antibody standards are highly purified and characterized recombinant IgG-1 monoclonal antibodies. They contain typical IgG glycosylation in a conserved Asn (#297) of the Fc region. The standards can be used as positive controls for PNGase F, IdeS protease, and other antibody-specific proteases.

Products:
Antibody Standard for PNGase F
Antibody Standard for Carboxypeptidase B

Mass Spec Standard Protein Extract (Yeast)

Coming Soon!

Mass Spec Standard Protein Extract (Human)

Coming Soon!

Deglycosylation Kit

Coming Soon!

Monoclonal Antibody Characterization Kit

Coming Soon!

Low pH Protein Digestion Kit

Coming Soon!