Chymotrypsin is a serine protease that selectively cleaves at the C-terminal of aromatic residues, particlarly those of Phenylalanine, Tyrosine and Tryptophan. Leucine, Methionine, Alanine, Aspartic acid and Glutamic acid may also be cleaved, but at a much slower rate. The product is highly purified with multiple chromatography steps and further treated withTosyl-L-lysyl-chloromethane hydrochloride (TLCK) to inactivate any trypsin activity which may be present without affecting chymotrypsin activity.
Chymotrypsin products from Edna have exceptional activity, specificity, stability and value in terms of cost per unit. Learn more in the Product Data Sheet and Brochure with product information, detailed usage protocols and sample results.
Chymotrypsin is a serine protease that selectively cleaves at the C-terminal of aromatic residues, particlarly those of Phenylalanine, Tyrosine and Tryptophan. The product is highly purified with multiple chromatography steps and further treated withTosyl-L-lysyl-chloromethane hydrochloride (TLCK) to inactivate any trypsin activity which may be present without affecting chymotrypsin activity.
What's In The Box:
The product includes a vial of the lyophilized enzyme and a vial of resuspension buffer (1 mM HCl), along with 2X chymotrypsin reaction buffer compatible with mass spectrometry systems.
Highlights:
Sourced from bovine pancreas and purified with multiple chromatography steps to >99% purity
High sequence coverage
Specificity improved by TLCK treatment to remove any trypsin activity
Supplied with 2X Reaction Buffer
Robust and reliable reactions
Use with EdnaPRO digestion improver (0.01–0.2% in reaction solution), a custom detergent for improved cleavage at hard-to-access sites
Suitable for digestion in both gel and solution
Compatible for co-digestion with other enzymes including PNGase F, Lysine-C, Glu-C, IdeS Protease, and more
Product Source
Bos Taurus pancreas
Unit Definition: One unit is defined as the amount of Chymotrypsin that will hydrolyze 1.0 μmol of BTEE per minute at pH 7.8 and 25°C.
Storage Temperature: -20 °C
Molecular Weight: 25 kDa
Activity: cleaves at the C-terminal of aromatic residues, particlarly those of Phenylalanine, Tyrosine and Tryptophan. Leucine, Methionine, Alanine, Aspartic acid and Glutamic acid may also be cleaved, but at a much slower rate.
Specific Activity: >80 BTEE units per mg
2x Chymotrypsin Reaction Buffer:
200 mM Tris-HCl
20 mM CaCl2
1x Chymotrypsin Resuspension Buffer:
1 mM HCl
Edna chymotrypsin products are well suited to a range of sequencing applications:
Peptide mapping
Protein identification
Protein fingerprinting
Biosimilar characterization
Cleavage reactions
View Product Data Sheet with Usage Protocol for Chymotrypsin, Lyophilized, Mass Spectrometry Grade
View Latest Certificate of Analysis for Chymotrypsin, Lyophilized, Mass Spectrometry Grade
View Product Brochure for Chymotrypsin, Lyophilized, Mass Spectrometry Grade
Modified Trypsin+EdnaPRO Digestion Improver Mix (Lyophilized)
Antibody Standard for Carboxypeptidase B - Coming Soon!
Extra Buffers and Reagents for Mass Spectrometry (for Trypsin, Chymotrypsin, PNGase F, and Carboxypeptidase B reactions)