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Chymotrypsin, Lyophilized, Mass Spectrometry Grade

Isolated from Bos Taurus pancreas, TLCK-treated

Chymotrypsin is a serine protease that selectively cleaves at the C-terminal of aromatic residues, particlarly those of Phenylalanine, Tyrosine and Tryptophan. Leucine, Methionine, Alanine, Aspartic acid and Glutamic acid may also be cleaved, but at a much slower rate. The product is highly purified with multiple chromatography steps and further treated withTosyl-L-lysyl-chloromethane hydrochloride (TLCK) to inactivate any trypsin activity which may be present without affecting chymotrypsin activity. 

Chymotrypsin products from Edna have exceptional activity, specificity, stability and value in terms of cost per unit. Learn more in the Product Data Sheet and Brochure with product information, detailed usage protocols  and sample results.

Product Information

Chymotrypsin is a serine protease that selectively cleaves at the C-terminal of aromatic residues, particlarly those of Phenylalanine, Tyrosine and Tryptophan.  The product is highly purified with multiple chromatography steps and further treated withTosyl-L-lysyl-chloromethane hydrochloride (TLCK) to inactivate any trypsin activity which may be present without affecting chymotrypsin activity.

What's In The Box:

The product includes a vial of the lyophilized enzyme and a vial of resuspension buffer (1 mM HCl), along with 2X chymotrypsin reaction buffer compatible with mass spectrometry systems.

Highlights:

Product Source

Bos Taurus pancreas

Properties and Storage

Unit Definition: One unit is defined as the amount of Chymotrypsin that will hydrolyze 1.0 μmol of BTEE per minute at pH 7.8 and 25°C.

Storage Temperature: -20 °C

Molecular Weight: 25 kDa

Activity: cleaves at the C-terminal of aromatic residues, particlarly those of Phenylalanine, Tyrosine and Tryptophan. Leucine, Methionine, Alanine, Aspartic acid and Glutamic acid may also be cleaved, but at a much slower rate.

Specific Activity: >80 BTEE units per mg

2x Chymotrypsin Reaction Buffer:

1x Chymotrypsin Resuspension Buffer:

Applications

Edna chymotrypsin products are well suited to a range of sequencing applications:

Supporting Documents

View Product Data Sheet with Usage Protocol for Chymotrypsin, Lyophilized, Mass Spectrometry Grade

View Latest Certificate of Analysis for Chymotrypsin, Lyophilized, Mass Spectrometry Grade

View Product Brochure for Chymotrypsin, Lyophilized, Mass Spectrometry Grade