Modified Trypsin, Frozen, Mass Spectrometry Grade
Isolated from Bos Taurus pancreas, TPCK-treated, Modified
Trypsin is a serine endopeptidase that selectively cleaves at the C-terminal of lysine and argnine residues. The product is highly purified with multiple chromatography steps and modified by reductive methylation to cap the ε-amino groups of lysine, and thus is extremely resistant to autolytic self-cleavage. The product is further treated with L-(tosylamido-2-phenyl) ethyl chloromethyl ketone (TPCK) to inactivate any chymotrypsin activity which may be present, without affecting trypsin activity.
Trypsin products from Edna have exceptional activity, specificity, stability and value in terms of cost per unit. Learn more in the Product Data Sheet and Brochure with product information, detailed usage protocols and sample results.
Trypsin is a serine endopeptidase that selectively cleaves at the C-terminal of lysine and argnine residues. The product is highly purified with multiple chromatography steps and further treated with L-(tosylamido-2-phenyl) ethyl chloromethyl ketone (TPCK) to inactivate any chymotrypsin-like activity. The product is also modified by reductive methylation to cap the ε-amino groups of lysine and prevent autolytic self-cleavage reactions. Edna Trypsin products are resistant to mild denaturing conditions such as 0.1% SDS, 1M urea or 10% acetonitrile.
What's In The Box:
The product includes a vial of the frozen enzyme in 50 mM acetic acid and a vial of dilution buffer (50 mM acetic acid) which can be used to further dilute the enzyme, along with 2X Trypsin reaction buffer compatible with mass spectrometry systems.
Sourced from bovine pancreas and purified with multiple chromatography steps to >99% purity
High sequence coverage
Specificity improved by TPCK treatment to remove any chymotryptic activity (reduced to <10 ATEE units per mg)
Resistant to mild denaturing conditions such as 0.1% SDS, 1M urea or 10% acetonitrile
Supplied with 2X Reaction Buffer
Robust and reliable reactions
Use with EdnaPRO digestion improver (0.01–0.2% in reaction solution), a custom detergent for improved cleavage at hard-to-access sites
Suitable for digestion in both gel and solution
Compatible for co-digestion with other enzymes including PNGase F, Lysine-C, Glu-C, IdeS Protease, and more
Bos Taurus pancreas
Properties and Storage
Unit Definition: One unit is the amount of enzyme required to produce a ΔA253 of 0.001 per minute at 30°C with the substrate Nα-benzoyl-L-arginine ethyl ester (BAEE).
Storage Temperature: -20 °C
Molecular Weight: 23.7kDa
Activity: Cleaves between Lys-Xaa and Arg-Xaa
Specific Activity: >19,000 BAEE units/mg
2x Trypsin Reaction Buffer:
100 mM Tris-HCl
40 mM CaCl2
1x Trypsin Dilution Buffer:
50 mM acetic acid
Edna trypsin products are well suited to a range of sequencing applications:
View Product Data Sheet with Usage Protocol for Trypsin, Frozen, Mass Spectrometry Grade
View Latest Certificate of Analysis for Trypsin, Frozen, Mass Spectrometry Grade
Antibody Standard for Carboxypeptidase B - Coming Soon!
Extra Buffers and Reagents for Mass Spectrometry (for Trypsin, Chymotrypsin, PNGase F, and Carboxypeptidase B reactions)