Home>Products>Mass Spectrometry>Modified Trypsin, Frozen, Mass Spectrometry Grade

Modified Trypsin, Frozen, Mass Spectrometry Grade

Isolated from Bos Taurus pancreas, TPCK-treated, Modified

Trypsin is a serine endopeptidase that selectively cleaves at the C-terminal of lysine and argnine residues.  The product is highly purified with multiple chromatography steps and modified by reductive methylation to cap the ε-amino groups of lysine, and thus is extremely resistant to autolytic self-cleavage. The product is further treated with L-(tosylamido-2-phenyl) ethyl chloromethyl ketone (TPCK) to inactivate any chymotrypsin activity which may be present, without affecting trypsin activity.

Trypsin products from Edna have exceptional activity, specificity, stability and value in terms of cost per unit. Learn more in the Product Data Sheet and Brochure with product information, detailed usage protocols  and sample results.

Product Information

Trypsin is a serine endopeptidase that selectively cleaves at the C-terminal of lysine and argnine residues.  The product is highly purified with multiple chromatography steps and further treated with L-(tosylamido-2-phenyl) ethyl chloromethyl ketone (TPCK) to inactivate any chymotrypsin-like activity.  The product is also modified by reductive methylation to cap the ε-amino groups of lysine and prevent autolytic self-cleavage reactions. Edna Trypsin products are resistant to mild denaturing conditions such as 0.1% SDS, 1M urea or 10% acetonitrile.

What's In The Box:

The product includes a vial of the frozen enzyme in 50 mM acetic acid and a vial of dilution buffer (50 mM acetic acid) which can be used to further dilute the enzyme, along with 2X Trypsin reaction buffer compatible with mass spectrometry systems.

Highlights:

Product Source

Bos Taurus pancreas

Properties and Storage

Unit Definition: One unit is the amount of enzyme required to produce a ΔA253 of 0.001 per minute at 30°C with the substrate Nα-benzoyl-L-arginine ethyl ester (BAEE).

Storage Temperature: -20 °C

Molecular Weight: 23.7kDa

Activity: Cleaves between Lys-Xaa and Arg-Xaa

Specific Activity: >19,000 BAEE units/mg

2x Trypsin Reaction Buffer:

1x Trypsin Dilution Buffer:

Applications

Edna trypsin products are well suited to a range of sequencing applications:

Supporting Documents

View Product Data Sheet with Usage Protocol for Trypsin, Frozen, Mass Spectrometry Grade

View Latest Certificate of Analysis for Trypsin, Frozen, Mass Spectrometry Grade

View Product Brochure for Trypsin, Frozen, Mass Spectrometry Grade