Modified Trypsin, Frozen, Mass Spectrometry Grade

Trypsin is a serine endopeptidase that selectively cleaves at the C-terminal of lysine and argnine residues. The product is highly purified with multiple chromatography steps and further treated with L-(tosylamido-2-phenyl) ethyl chloromethyl ketone (TPCK) to inactivate any chymotrypsin-like activity. The product is also modified by reductive methylation to cap the ε-amino groups of lysine and prevent autolytic self-cleavage reactions. Edna Trypsin products are resistant to mild denaturing conditions such as 0.1% SDS, 1M urea or 10% acetonitrile.

What's In The Box:

The product includes a vial of the frozen enzyme in 50 mM acetic acid and a vial of dilution buffer (50 mM acetic acid) which can be used to further dilute the enzyme, along with 2X Trypsin reaction buffer compatible with mass spectrometry systems.

Highlights:

• Sourced from bovine pancreas and purified with multiple chromatography steps to >99% purity

• High sequence coverage

• Specificity improved by TPCK treatment to remove any chymotryptic activity (reduced to <10 ATEE units per mg)

• Resistant to mild denaturing conditions such as 0.1% SDS, 1M urea or 10% acetonitrile

• Supplied with 2X Reaction Buffer

• Robust and reliable reactions

• Use with EdnaPRO digestion improver (0.01–0.2% in reaction solution), a custom detergent for improved cleavage at hard-to-access sites

• Suitable for digestion in both gel and solution

• Compatible for co-digestion with other enzymes including PNGase F, Lysine-C, Glu-C, IdeS Protease, and more

Product Source

Bos Taurus pancreas

Unit Definition: One unit is the amount of enzyme required to produce a ΔA253 of 0.001 per minute at 30°C with the substrate Nα-benzoyl-L-arginine ethyl ester (BAEE).

Storage Temperature: -20 °C

Molecular Weight: 23.7kDa

Activity: Cleaves between Lys-Xaa and Arg-Xaa

Specific Activity: >19,000 BAEE units/mg

2x Trypsin Reaction Buffer:

• 100 mM Tris-HCl

• 40 mM CaCl₂

1x Trypsin Dilution Buffer:

• 50 mM acetic acid

Edna trypsin products are well suited to a range of sequencing applications:

1. Peptide mapping

2. Protein identification

3. Protein fingerprinting

4. Biosimilar characterization

5. Cleavage reactions

View Product Data Sheet with Usage Protocol for Trypsin, Frozen, Mass Spectrometry Grade

View Latest Certificate of Analysis for Trypsin, Frozen, Mass Spectrometry Grade

View Product Brochure for Trypsin, Frozen, Mass Spectrometry Grade

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