Carboxypeptidase B, Ultrapure, Frozen

Carboxypeptidase B (CPB) is a pancreatic endo-peptidase that cleaves C-terminal basic Arg and Lys residues. It is routinely used in characterization of basic variants of monoclonal antibodies, as well as in production processes for molecules such as insulin. It is also used in mAb production processes for reducing the charge heterogeneity of the mAb material.

CPB is typically isolated from bovine, porcine, rat or human pancreas. Edna CPB is produced by expressing the CPB gene from Rattus norvegicus in E. coli, allowing for expression of the rat enzyme without any of the contaminants associated with pancreatic isolation processes, such as Carboxypeptidase A and trypsin, which can significantly reduce the consistency and specificity of the enzyme product. The Edna process expresses the enzyme as an unfolded precursor, followed by in-vitro refolding and prosequence cleavage. These are followed by multiple chromatography steps to ensure that all the enzyme present in the vial is in its folded, maximally active form.

What's In The Box:

The product includes a vial of the formulated frozen enzyme as per the selected formulation (standard/glycerol-free), and vial of digestion reaction buffer, to be used as per the protocols in the attached Product Data Sheet.

Highlights:

• Cloned from Rattus norvegicus, recombinantly produced in E. coli and purified to >99% purity

• Produced as a tag-free pro-enzyme with in-vitro refolding, activation, and multi-step purification

• Available as active enzyme with non-dialyzable zinc ion incorporated in-vitro

• Available in both lyophilized and frozen formulations

• Extensively tested in mAb characterization for removal of Lys residues in basic charge variants

• Guaranteed free from impurities like Carboxypeptidase A often found in non-recombinant products

Product Source:

An E. coli strain that carries the CPB gene from Rattus norvegicus.

Unit Definition: One unit is defined as the amount of enzyme required to hydrolyze 1 µmol of Hippuryl-L-Arg per minute at 30 °C. pH 9.5.

Storage Temperature: -20 °C

Molecular Weight: 35 kDa

Activity: Removal of C-terminal Lys and Arg from proteins

Concentration: 2.8 mg/mL⁺²

Specific Activity: 200 Units/mg

Maximum Temperature: 60 °C

Catalytic pH Range: 5.0 - 12.0

10X CPB Digestion Reaction Buffer:

• 250 mM Tris HCl

• 1 M NaCl

pH 8.0

Edna CPB products are well suited to a range of applications:

1. Isoform characterization

2. In-process applications for molecules such as insulin

3. Reducing charge heterogeneity of mAbs

View Product Data Sheet with Usage Protocol for Carboxypeptidase B, Ultrapure, Frozen

View Latest Certificate of Analysis for Carboxypeptidase B, Ultrapure, Frozen

View Product Brochure for Carboxypeptidase B, Ultrapure

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• Antibody Standard for PNGase F

• Modified Trypsin, Frozen, Mass Spectrometry Grade

• Modified Trypsin, Lyophilized, Mass Spectrometry Grade

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• Chymotrypsin, Lyophilized, Mass Spectrometry Grade

• Chymotrypsin+EdnaPRO Digestion Improver Mix (Lyophilized)

• EdnaPRO Digestion Improver

• Antibody Standard for Carboxypeptidase B - Coming Soon!

• Extra Buffers and Reagents for Mass Spectrometry (for Trypsin, Chymotrypsin, PNGase F, and Carboxypeptidase B reactions)