EdnaQuick PNGase F Reaction Mix

PNGase F, also known as N-Glycosidase F, is an amidase which cleaves N-linked oligosaccharides from glycoproteins. The cleavage acts between the innermost GlcNAc and asparagine residues of high mannose, hybrid and complex oligosaccharides. PNGase F is active under both native and denaturing conditions. The product is recombinant and totally free of contaminating endoglycosidases.

EdnaQuick PNGase F Reaction Mix Rapid is an improved formulation and reaction mix facilitating quick deglycosylation of antibodies and fusion proteins in mere minutes instead of >4 hours.  Processing time is significantly reduced without compromising on reaction sensitivity, reliability and reproducibility.

What's In The Box:

The product includes a vial of the Quick Action frozen enzyme, along with a vial of customized 5X Quick Action reaction buffer.

Highlights:

• Recombinant enzyme completely free from contaminating endoglycosidases F1, F2 and F3

• Includes customized Quick Action reaction buffer

• Includes optimized protocol for deglycosylation in 10-20 min instead of hours

• Ideal for fastest, easiest removal of N-linked oligosaccharides from glycoproteins

Enzyme Source:

An E. coli strain that carries the PNGase F gene from Elizabethkingia miricola

Unit Definition: One unit is defined as the amount of enzyme required to remove > 95% of the carbohydrate from 10 µg of denatured RNase B in 1 hour at 37°C in a total reaction volume of 10 µl. 

Storage Temperature: -20 °C

Molecular Weight: 36 kDa

Activity: Removal of N-linked oligosaccharides from glycoproteins

Edna PNGase F products are well suited to a range of applications:

1. Mass spectrometry-based N-glycan analysis

2. Glycoprotein analysis

3. Biosimilar characterization studies

4. Removal of N-glycans for other applications

View Product Data Sheet with Usage Protocol for EdnaQuick PNGase F Reaction Mix

View Latest Certificate of Analysis for EdnaQuick PNGase F Reaction Mix

View Product Brochure for PNGase F and EdnaQuick PNGase F Reaction Mix

• PNGase F

• PNGase F, Glycerol-free

• PNGase F, Glycerol-free and EDTA-free

• Antibody Standard for PNGase F

• Modified Trypsin, Frozen, Mass Spectrometry Grade

• Modified Trypsin, Lyophilized, Mass Spectrometry Grade

• Modified Trypsin+EdnaPRO Digestion Improver Mix (Lyophilized)

• Chymotrypsin, Lyophilized, Mass Spectrometry Grade

• Chymotrypsin+EdnaPRO Digestion Improver Mix (Lyophilized)

• EdnaPRO Digestion Improver

• Carboxypeptidase B, Ultrapure, Frozen

• Carboxypeptidase B, Ultrapure, Frozen, Glycerol-free

• Antibody Standard for Carboxypeptidase B - Coming Soon!

• Extra Buffers and Reagents for Mass Spectrometry (for Trypsin, Chymotrypsin, PNGase F, and Carboxypeptidase B reactions)