PNGase F

PNGase F, also known as N-Glycosidase F, is an amidase which cleaves N-linked oligosaccharides from glycoproteins. The cleavage acts between the innermost GlcNAc and asparagine residues of high mannose, hybrid and complex oligosaccharides. PNGase F is active under both native and denaturing conditions. The product is recombinant and totally free of contaminating endoglycosidases.

What's In The Box:

The product includes a vial of the formulated frozen enzyme as per the selected formulation (standard/glycerol-free/glycerol-free and EDTA-free), a vial of reaction buffer, a vial of glycoprotein denaturing buffer and a vial of NP-40 reagent, to be used as per the protocols in the attached Product Data Sheet.

Highlights:

• Available in standard, glycerol-free and glycerol- and EDTA-free buffers offering a range of choices for compatibility with further analysis tools

• Completely free from contaminating endoglycosidases F1, F2 and F3

• EdnaQuick PNGase F Reaction Mix is available with unique reagents and protocol for total deglycosylation in 20 min instead of hours

• Includes complete set of reaction buffer, denaturing buffer and NP-40 for hassle-free reactions

• Ideal for fastest, easiest removal of N-linked oligosaccharides from glycoproteins

Product Source:

An E. coli strain that carries the PNGase F gene from Elizabethkingia miricola

Unit Definition: One unit is defined as the amount of enzyme required to remove > 95% of the carbohydrate from 10 µg of denatured RNase B in 1 hour at 37°C in a total reaction volume of 10 µl.

Storage Temperature: -20 °C

Molecular Weight: 36 kDa

Activity: Removal of N-linked oligosaccharides from glycoproteins

Concentration: 500 Units/µl

10X PNGase F Reaction Buffer:

• 500 mM Sodium Phosphate

pH 7.5

10X Glycoprotein Denaturing Buffer:

• 5% SDS

• 400 mM DTT

1X NP-40:

• 1% NP-40 in MilliQ water

Edna PNGase F products are well suited to a range of applications:

1. Mass spectrometry-based N-glycan analysis

2. Glycoprotein analysis

3. Biosimilar characterization studies

4. Removal of N-glycans for other applications

View Product Data Sheet with Usage Protocol for PNGase F

View Latest Certificate of Analysis for PNGase F

View Product Brochure for PNGase F and EdnaQuick PNGase F Reaction Mix

• EdnaQuick PNGase F Reaction Mix

• Antibody Standard for PNGase F

• Modified Trypsin, Frozen, Mass Spectrometry Grade

• Modified Trypsin, Lyophilized, Mass Spectrometry Grade

• Modified Trypsin+EdnaPRO Digestion Improver Mix (Lyophilized)

• Chymotrypsin, Lyophilized, Mass Spectrometry Grade

• Chymotrypsin+EdnaPRO Digestion Improver Mix (Lyophilized)

• EdnaPRO Digestion Improver

• Carboxypeptidase B, Ultrapure, Frozen

• Carboxypeptidase B, Ultrapure, Frozen, Glycerol-free

• Antibody Standard for Carboxypeptidase B - Coming Soon!

• Extra Buffers and Reagents for Mass Spectrometry (for Trypsin, Chymotrypsin, PNGase F, and Carboxypeptidase B reactions)