Cloned from Elizabethkingia miricola, overexpressed in E. coli
Available in three formulations: Standard, Glycerol-free, or Glycerol- and EDTA-free
PNGase F, also known as N-Glycosidase, is an amidase which cleaves N-linked oligosaccharides from glycoproteins. The cleavage acts between the innermost GlcNAc and asparagine residues of high mannose, hybrid and complex oligosaccharides. PNGase F is active under both native and denaturing conditions.
PNGase F products from Edna have exceptional activity, specificity, stability and value in terms of cost per unit. The product is recombinant and totally free of contaminating endoglycosidases. The enzyme is also available in Glycerol-free and Glycerol- and EDTA-free formulations. Learn more in the Product Data Sheet and Brochure with product information, detailed usage protocols and sample gel results.
PNGase F, also known as N-Glycosidase F, is an amidase which cleaves N-linked oligosaccharides from glycoproteins. The cleavage acts between the innermost GlcNAc and asparagine residues of high mannose, hybrid and complex oligosaccharides. PNGase F is active under both native and denaturing conditions. The product is recombinant and totally free of contaminating endoglycosidases.
What's In The Box:
The product includes a vial of the formulated frozen enzyme as per the selected formulation (standard/glycerol-free/glycerol-free and EDTA-free), a vial of reaction buffer, a vial of glycoprotein denaturing buffer and a vial of NP-40 reagent, to be used as per the protocols in the attached Product Data Sheet.
Available in standard, glycerol-free and glycerol- and EDTA-free buffers offering a range of choices for compatibility with further analysis tools
Completely free from contaminating endoglycosidases F1, F2 and F3
EdnaQuick PNGase F Reaction Mix is available with unique reagents and protocol for total deglycosylation in 20 min instead of hours
Includes complete set of reaction buffer, denaturing buffer and NP-40 for hassle-free reactions
Ideal for fastest, easiest removal of N-linked oligosaccharides from glycoproteins
An E. coli strain that carries the PNGase F gene from Elizabethkingia miricola
Properties and Storage
Unit Definition: One unit is defined as the amount of enzyme required to remove > 95% of the carbohydrate from 10 µg of denatured RNase B in 1 hour at 37°C in a total reaction volume of 10 µl.
Storage Temperature: -20 °C
Molecular Weight: 36 kDa
Activity: Removal of N-linked oligosaccharides from glycoproteins
Concentration: 500 Units/µl
10X PNGase F Reaction Buffer:
500 mM Sodium Phosphate
10X Glycoprotein Denaturing Buffer:
400 mM DTT
1% NP-40 in MilliQ water
Edna PNGase F products are well suited to a range of applications:
Mass spectrometry-based N-glycan analysis
Biosimilar characterization studies
Removal of N-glycans for other applications
Antibody Standard for Carboxypeptidase B - Coming Soon!
Extra Buffers and Reagents for Mass Spectrometry (for Trypsin, Chymotrypsin, PNGase F, and Carboxypeptidase B reactions)