EdnaQuick Ligation Kit with T4 DNA Ligase

T4 DNA Ligase catalyzes the formation of phosphodiester bonds between 5' and 3' termini in double stranded DNA using ATP as a cofactor. The enzyme can be used for ligation of both blunt and sticky ends as well as single-stranded DNA nick repair. Edna T4 DNA Ligase products are recombinantly produced in E. coli and thus are free from contaminating nucleases, offering robust and reliable ligation activity.

The Rapid /Blunt-End Ligation Kit comes with custom ligation buffer and PEG-4000, enabling 10 minute ligation reactions at room temperature. Ligation efficiency is equal to that obtained by normal 1-2 hour ligations with T4 DNA Ligase under standard conditions for sticky-end ligation, and significantly enhanced for blunt-end ligation.

What's In The Box:

The product includes one vial of recombinant enzyme, one vial of 5x custom rapid ligation buffer and 1 vial of PEG-4000 solution, along with detailed protocol.

Highlights:

• Guaranteed 10 minute ligation reactions for even the most challenging blunt-ends and combination digests

• T4 DNA Ligase cloned from Bacteriophage T4 and recombinantly produced in E. coli with >99% purity

• Detergent free formulations compatible with post-processing applications

• Ideal for cloning of restriction enzyme generated DNA fragments and PCR products

Product Source:

An E. coli strain that carries the T4 DNA Ligase gene from Bacteriophage T4.

Unit Definition: One unit is defined as the amount of enzyme required to give 50% ligation of HindIII fragments of DNA from Bacteriophage λ (5´ DNA termini concentration of 0.12 µM, 300- µg/ml) in a total reaction volume of 20 μl in 30 minutes at 16°C in 1x T4 DNA Ligase Reaction Buffer. 

Storage Temperature: -20 °C

Enzyme Molecular Weight: 68 kDa

Enzyme Heat Inactivation: >65 °C for 15 minutes 

Edna's Rapid/Blunt-End Ligation Kit is well suited to a range of molecular biology applications:

1. Cloning of restriction enzyme generated DNA fragments

2. Cloning of PCR products

3. Joining of blunt-ended DNA

4. Joining of double-stranded oligonucleotide linkers to DNA

5. Site-directed mutagenesis

6. Nick repair in duplex DNA, RNA or DNA/RNA hybrids

7. Self-circularization of linear DNA

View Product Data Sheet with Usage Protocol for Rapid/Blunt-End Ligation Kit with T4 DNA Ligase

View Latest Certificate of Analysis for Rapid/Blunt-End Ligation Kit with T4 DNA Ligase

View Product Brochure for T4 DNA Ligase and Rapid/Blunt-End Ligation Kit

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