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EdnaQuick Ligation Kit with T4 DNA Ligase

Contains enzyme T4 DNA Ligase cloned from Bacteriophage T4 , overexpressed in E. coli
With 10x rapid ligation buffer, PEG-4000 and custom protocol for 10-minute ligation of sticky-ends, blunt-ends and double/multiple digests

T4 DNA Ligase is routinely used for ligation reactions in molecular biology. T4 DNA Ligase products from Edna have exceptional activity, stability and value in terms of cost per unit. Edna's Rapid Ligation Kit contains the enzyme along with a custom ligation buffer formulation with PEG-4000, guaranteeing 10 minute ligation reactions at room temperature for even the most challenging blunt-ends and combination digests.

Learn more in our Product Data Sheet and Brochure which includes product information, detailed usage protocols and sample gel results.

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Product Information

T4 DNA Ligase catalyzes the formation of phosphodiester bonds between 5' and 3' termini in double stranded DNA using ATP as a cofactor. The enzyme can be used for ligation of both blunt and sticky ends as well as single-stranded DNA nick repair. Edna T4 DNA Ligase products are recombinantly produced in E. coli and thus are free from contaminating nucleases, offering robust and reliable ligation activity.

The Rapid /Blunt-End Ligation Kit comes with custom ligation buffer and PEG-4000, enabling 10 minute ligation reactions at room temperature. Ligation efficiency is equal to that obtained by normal 1-2 hour ligations with T4 DNA Ligase under standard conditions for sticky-end ligation, and significantly enhanced for blunt-end ligation.

What's In The Box:

The product includes one vial of recombinant enzyme, one vial of 5x custom rapid ligation buffer and 1 vial of PEG-4000 solution, along with detailed protocol.

Highlights:

  • Guaranteed 10 minute ligation reactions for even the most challenging blunt-ends and combination digests

  • T4 DNA Ligase cloned from Bacteriophage T4 and recombinantly produced in E. coli with >99% purity

  • Detergent free formulations compatible with post-processing applications

  • Ideal for cloning of restriction enzyme generated DNA fragments and PCR products

Product Source:

An E. coli strain that carries the T4 DNA Ligase gene from Bacteriophage T4.

Properties and Storage

Unit Definition: One unit is defined as the amount of enzyme required to give 50% ligation of HindIII fragments of DNA from Bacteriophage λ (5´ DNA termini concentration of 0.12 µM, 300- µg/ml) in a total reaction volume of 20 μl in 30 minutes at 16°C in 1x T4 DNA Ligase Reaction Buffer.

Storage Temperature: -20 °C

Enzyme Molecular Weight: 68 kDa

Enzyme Heat Inactivation: >65 °C for 15 minutes

Applications

Edna's Rapid/Blunt-End Ligation Kit is well suited to a range of molecular biology applications:

  1. Cloning of restriction enzyme generated DNA fragments

  2. Cloning of PCR products

  3. Joining of blunt-ended DNA

  4. Joining of double-stranded oligonucleotide linkers to DNA

  5. Site-directed mutagenesis

  6. Nick repair in duplex DNA, RNA or DNA/RNA hybrids

  7. Self-circularization of linear DNA

Supporting Documents

View Product Data Sheet with Usage Protocol for Rapid/Blunt-End Ligation Kit with T4 DNA Ligase

View Latest Certificate of Analysis for Rapid/Blunt-End Ligation Kit with T4 DNA Ligase

View Product Brochure for T4 DNA Ligase and Rapid/Blunt-End Ligation Kit

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