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Taq DNA Polymerase with 10x Buffer (Mg-free) and MgCl2

Cloned from Thermus aquaticus, overexpressed in E. coli

Taq DNA Polymerase is routinely used for PCR applications in molecular biology. Taq products from Edna have exceptional activity, stability and value in terms of cost per unit. This Mg-free formulation comes with separate MgCl2 solution for easy optimization of Mg2+ concentrations in the reaction.

Learn more in the Product Data Sheet and Brochure which includes product information, detailed usage protocols and sample gel results.

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Product Information

Taq DNA Polymerase is a thermostable DNA polymerase that has 5´→ 3´ polymerase activity and 5´ flap endonuclease activity. Edna Taq products are recombinantly produced in E. coli and thus are highly pure and offer robust and reliable PCR activity.

What's In The Box:

The product includes a vial of the formulated recombinant enzyme along with 10X Taq reaction buffer (Mg-free) which is detergent-free and designed to be compatible with existing assay systems. This product also includes a separate vial of 25 mM MgCl2 solution.


  • Isolated from a recombinant source

  • The enzyme is free of any tags

  • Supplied with 10X Reaction Buffer

  • Robust and reliable reactions

  • Tolerates a wide range of templates

  • Incorporates dUTP, dITP and fluorescently-labeled nucleotides

  • An ideal tool for amplifying DNA of length <5 kb

Product Source

An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus.

Properties and Storage

Unit Definition: One unit is defined as the amount of enzyme that will incorporate 15 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C.

Storage Temperature: -20 C

Molecular Weight: 94 kDa

Activity: 5'-3' exonuclease (not 3'-5') resulting in single-base 3' overhangs

Heat Inactivation: None

10x Taq Reaction Buffer (Mg-free):

  • 100 mM Tris-HCl

  • 500 mM KCl

(pH 8.3)

Supplied with separate vial of 25 mM MgCl2 solution.


Edna Taq DNA Polymerase products are well suited to a range of molecular biology applications:

  1. Polymerase Chain Reaction (PCR)

  2. Primer Extension

  3. Colony PCR

  4. Microarray Analysis

  5. DHPLC

Supporting Documents

View Product Data Sheet with Usage Protocol for Taq DNA Polymerase with 10x Buffer (Mg-free) and MgCl2

View Latest Certificate of Analysis for Taq DNA Polymerase with 10x Buffer (Mg-free) and MgCl2

View Product Brochure for Taq DNA Polymerase with 10x Buffer (Mg-free) and MgCl2