Taq DNA Polymerase with 10x Buffer (Mg-free) and MgCl2

Taq DNA Polymerase is a thermostable DNA polymerase that has 5´→ 3´ polymerase activity and 5´ flap endonuclease activity.  Edna Taq products are recombinantly produced in E. coli and thus are highly pure and offer robust and reliable PCR activity.

What's In The Box:

The product includes a vial of the formulated recombinant enzyme along with 10X Taq reaction buffer (Mg-free) which is detergent-free and designed to be compatible with existing assay systems.  This product also includes a separate vial of 25 mM MgCl2 solution. 

Highlights:

• Isolated from a recombinant source

• The enzyme is free of any tags

• Supplied with 10X Reaction Buffer

• Robust and reliable reactions

• Tolerates a wide range of templates

• Incorporates dUTP, dITP and fluorescently-labeled nucleotides

• An ideal tool for amplifying DNA of length <5 kb

Product Source

An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus.

Unit Definition: One unit is defined as the amount of enzyme that will incorporate 15 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C.  

Storage Temperature: -20 C

Molecular Weight: 94 kDa

Activity: 5'-3' exonuclease (not 3'-5') resulting in single-base 3' overhangs

Heat Inactivation: None

10x Taq Reaction Buffer (Mg-free):

• 100 mM Tris-HCl

• 500 mM KCl

(pH 8.3)

Supplied with separate vial of 25 mM MgCl2 solution.

Edna Taq DNA Polymerase products are well suited to a range of molecular biology applications:

1. Polymerase Chain Reaction (PCR)

2. Primer Extension

3. Colony PCR

4. Microarray Analysis

5. DHPLC

View Product Data Sheet with Usage Protocol for Taq DNA Polymerase  with 10x Buffer (Mg-free) and MgCl2

View Latest Certificate of Analysis for Taq DNA Polymerase  with 10x Buffer (Mg-free) and MgCl2

View Product Brochure for Taq DNA Polymerase with 10x Buffer (Mg-free) and MgCl2

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