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High-fidelity Pfu DNA Polymerase with 10x Buffer

Cloned from Pyrococcus furiosus, overexpressed in E. coli

Pfu DNA Polymerase is routinely used for PCR applications in molecular biology. Due to its proofreading activity, Pfu DNA polymerase is used for high fidelity transcription with greatly reduced error rate compared to standard Taq DNA polymerase products.

Pfu products from Edna have exceptional activity, stability and value in terms of cost per unit. Learn more in our Product Data Sheet and Brochure which includes product information, detailed usage protocols and sample gel results.

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Products Offered

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Product Information

Pfu DNA Polymerase is a thermostable DNA polymerase that has 5´→ 3´ polymerase activity and 5´ flap endonuclease activity. The enzyme also exhibits 3´→5´ exonuclease (proofreading) activity. Base misinsertions are rapidly removed by the polymerase, making it a good option for applications where high-fidelity error-free synthesis is required. Edna Pfu products are recombinantly produced in E. coli and thus are highly pure and offer robust and reliable PCR activity.

What's In The Box:

The product includes a vial of the formulated recombinant enzyme along with 10X Pfu reaction buffer which is detergent-free and designed to be compatible with existing assay systems.


  • Isolated from a recombinant source

  • The enzyme is free of any tags

  • Supplied with 10X Reaction Buffer

  • Robust and reliable reactions

  • Tolerates a wide range of templates

  • Incorporates dUTP, dITP and fluorescently-labeled nucleotides

  • An ideal tool for amplifying DNA of length <5 kb

Product Source

An E. coli strain that carries the Pfu DNA Polymerase gene from Pyrococcus furiosus.

Properties and Storage

Unit Definition: : One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nanomoles of dNTPs into acid insoluble material in 30 minutes at 75°C.

Storage Temperature: -20 C

Molecular Weight: 92 kDa

Activity: 5'-3' exonuclease (not 3'-5') resulting in single-base 3' overhangs. The enzyme also exhibits 3´→ 5´ exonuclease (proofreading) activity.

Heat Inactivation: None

10x Pfu Reaction Buffer:

  • 200 mM Tris-HCl

  • 100 mM KCl

  • 100 mM (NH₄)₂SO₄

  • 10 mM MgSO4

  • 1% Triton X-100

(pH 8.8)


Edna Pfu DNA Polymerase products are well suited to a range of molecular biology applications:

  1. Polymerase Chain Reaction (PCR)

  2. Primer Extension

  3. Colony PCR

  4. Microarray Analysis

  5. DHPLC

Supporting Documents

View Product Data Sheet with Usage Protocol for High-fidelity Pfu DNA Polymerase with 10x Buffer

View Latest Certificate of Analysis for High-fidelity Pfu DNA Polymerase with 10x Buffer

View Product Brochure for High-fidelity Pfu DNA Polymerase with 10x Buffer