Taq DNA Polymerase with 10x Buffer

Taq DNA Polymerase is a thermostable DNA polymerase that has 5´→ 3´ polymerase activity and 5´ flap endonuclease activity. Edna Taq products are recombinantly produced in E. coli and thus are highly pure and offer robust and reliable PCR activity.

What's In The Box:

The product includes a vial of the formulated recombinant enzyme along with 10X Taq reaction buffer which is detergent-free and designed to be compatible with existing assay systems.

Highlights:

• Isolated from a recombinant source

• The enzyme is free of any tags

• Supplied with 10X Reaction Buffer

• Robust and reliable reactions

• Tolerates a wide range of templates

• Incorporates dUTP, dITP and fluorescently-labeled nucleotides

• An ideal tool for amplifying DNA of length <5 kb

Product Source

An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus.

Unit Definition: One unit is defined as the amount of enzyme that will incorporate 15 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C.

Storage Temperature: -20 C

Molecular Weight: 94 kDa

Activity: 5'-3' exonuclease (not 3'-5') resulting in single-base 3' overhangs

Heat Inactivation: None

10x Taq Reaction Buffer:

• 100 mM Tris-HCl

• 500 mM KCl

• 15 mM MgCl₂

(pH 8.3)

Edna Taq DNA Polymerase products are well suited to a range of molecular biology applications:

1. Polymerase Chain Reaction (PCR)

2. Primer Extension

3. Colony PCR

4. Microarray Analysis

5. DHPLC

View Product Data Sheet with Usage Protocol for Taq DNA Polymerase with 10x Buffer

View Latest Certificate of Analysis for Taq DNA Polymerase with 10x Buffer

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