T4 DNA Ligase

T4 DNA Ligase catalyzes the formation of phosphodiester bonds between 5' and 3' termini in double stranded DNA using ATP as a cofactor. The enzyme can be used for ligation of both blunt and sticky ends as well as single-stranded DNA nick repair.

Edna T4 DNA Ligase products are recombinantly produced in E. coli and thus are free from contaminating nucleases, offering robust and reliable ligation activity.

What's In The Box:

The product includes one vial of recombinant enzyme at standard or high concentration, along with one vial of 10x ligation buffer.

1X Standard Ligation Buffer:

50 mM Tris-HCl
10 mM MgCl₂
1 mM ATP
10 mM DTT
pH 7.5 @ 25°C

Highlights:

• Cloned from Bacteriophage T4 and recombinantly produced in E. coli with >99% purity

• Available at standard or high concentration for when minimal dilution is critical

• Detergent free formulations compatible with post-processing applications

• Ideal for cloning of restriction enzyme generated DNA fragments and PCR products

Product Source:

An E. coli strain that carries the T4 DNA Ligase gene from Bacteriophage T4.

Unit Definition: One unit is defined as the amount of enzyme required to give 50% ligation of HindIII fragments of DNA from Bacteriophage λ (5´ DNA termini concentration of 0.12 µM, 300- µg/ml) in a total reaction volume of 20 μl in 30 minutes at 16°C in 1x T4 DNA Ligase Reaction Buffer.

Concentration:

• Standard Concentration: 400 CEU/μl

• High Concentration: 2,000 CEU/μl

Storage Temperature: -20 °C

Molecular Weight: 68 kDa

Heat Inactivation: >65 °C for 15 minutes

Edna T4 DNA Ligase products are well suited to a range of molecular biology applications:

1. Cloning of restriction enzyme generated DNA fragments

2. Cloning of PCR products

3. Joining of double-stranded oligonucleotide linkers to DNA

4. Site-directed mutagenesis

5. Nick repair in duplex DNA, RNA or DNA/RNA hybrids

6. Self-circularization of linear DNA

View Product Data Sheet with Usage Protocol for T4 DNA Ligase, Standard Concentration (400 CEU/μL)

View Product Data Sheet with Usage Protocol for T4 DNA Ligase, High Concentration (2,000 CEU/μL)

View Latest Certificate of Analysis for T4 DNA Ligase, Standard Concentration (400 CEU/μL)

View Latest Certificate of Analysis for T4 DNA Ligase, High Concentration (2,000 CEU/μL)

View Product Brochure for T4 DNA Ligase and Rapid/Blunt-End Ligation Kit

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