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T4 DNA Ligase

Cloned from Bacteriophage T4, overexpressed in E. coli
Available in standard and high concentration formulations with 10x ligation buffer

T4 DNA Ligase is routinely used for ligation reactions in molecular biology. T4 DNA Ligase products from Edna have exceptional activity, stability and value in terms of cost per unit. We also offer custom kits for rapid and blunt-end ligations.

Learn more in our Product Data Sheet and Brochure which includes product information, detailed usage protocols and sample gel results.

Product Information

T4 DNA Ligase catalyzes the formation of phosphodiester bonds between 5' and 3' termini in double stranded DNA using ATP as a cofactor. The enzyme can be used for ligation of both blunt and sticky ends as well as single-stranded DNA nick repair. 

Edna T4 DNA Ligase products are recombinantly produced in E. coli and thus are free from contaminating nucleases, offering robust and reliable ligation activity.

What's In The Box:

The product includes one vial of recombinant enzyme at standard or high concentration, along with one vial of 10x ligation buffer.

1X Standard Ligation Buffer:

50 mM Tris-HCl
10 mM MgCl2
1 mM ATP
10 mM DTT
pH 7.5 @ 25°C

Highlights:

Product Source:

An E. coli strain that carries the T4 DNA Ligase gene from Bacteriophage T4.

Properties and Storage

Unit Definition: One unit is defined as the amount of enzyme required to give 50% ligation of HindIII fragments of DNA from Bacteriophage λ (5´ DNA termini concentration of 0.12 µM, 300- µg/ml) in a total reaction volume of 20 μl in 30 minutes at 16°C in 1x T4 DNA Ligase Reaction Buffer. 

Concentration

Storage Temperature: -20 °C

Molecular Weight: 68 kDa

Heat Inactivation: >65 °C for 15 minutes 

Applications

Edna T4 DNA Ligase products are well suited to a range of molecular biology applications:

Supporting Documents

View Product Data Sheet with Usage Protocol for T4 DNA Ligase, Standard Concentration (400 CEU/μL)

View Product Data Sheet with Usage Protocol for T4 DNA Ligase, High Concentration (2,000 CEU/μL)

View Latest Certificate of Analysis for T4 DNA Ligase, Standard Concentration (400 CEU/μL)

View Latest Certificate of Analysis for T4 DNA Ligase, High Concentration (2,000 CEU/μL)

View Product Brochure for T4 DNA Ligase and Rapid/Blunt-End Ligation Kit