Ultra-RNase A, Recombinant
Cloned from Bos Taurus, overexpressed in E. coli
RNase A is an endoribonuclease routinely used for removal of RNA during DNA isolation procedures. RNase A products from Edna are 100% recombinant and free of any contaminating nucleases.
Through extensive purification and refolding steps, Edna’s Ultra-RNase A has 2-3x the activity in Kunitz units per mg compared to competing products in the market extracted from bovine pancreas.
RNase A is an endoribonuclease routinely used for removal of RNA during DNA isolation procedures.
RNase A products from Edna are 100% recombinant and free of other nucleases. The product is well-suited to specialty applications such as gene therapy research as the risk of introducing pathogens is minimized.
Through extensive purification and refolding steps, Edna’s Ultra-RNase A has 3x the activity in Kunitz units per mg compared to competing products in the market extracted from bovine pancreas.
What's In The Box:
The product includes one vial of recombinant enzyme.
Cloned from bovine pancreas and recombinantly produced in E. coli and purified to >99% purity
Refolded in-vitro and purified to remove non-active forms
3x activity in Kunitz units/mg compared to competing non-recombinant products
Well suited for routine molecular biology applications as well as specialized work that requires bovine-free products
An E. coli strain that carries the RNase A gene from Bos Taurus.
Properties and Storage
Unit Definition: One unit of Ribonuclease A activity will cause a decrease of 100% per minute in the value of E0 – Ef at pH 5.0 at 25°C for the reaction Ribonucleic acid + water --> Oligonucleotides in the presence of RNase A, where E is the absorbance at 300 nm with path length of 1 cm.
Concentration: 1 mg/mL
Storage Temperature: -20 °C
Molecular Weight: 13.7 kDa
Plasmid and genomic DNA preparation
Removal of RNA from recombinant protein preparations
In preparing DNA for real time PCR
In DNA preparations for sequencing
In addition, Edna Ultra-RNase A products have a unique range of advantages:
Rigorously tested against nonspecific nuclease and protease activity
Second-step purification post-refolding to ensure that only maximally active refolded protein is present in the final formulation
Useful for specialty applications requiring bovine-free products such as gene therapy research
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