RNase A is an endoribonuclease routinely used for removal of RNA during DNA isolation procedures. RNase A products from Edna are 100% recombinant and free of any contaminating nucleases.
Through extensive purification and refolding steps, Edna’s Ultra-RNase A has 2-3x the activity in Kunitz units per mg compared to competing products in the market extracted from bovine pancreas.
Learn more in our Product Data Sheet and Brochure which includes product information, detailed usage protocols and sample gel results.
RNase A is an endoribonuclease routinely used for removal of RNA during DNA isolation procedures.
RNase A products from Edna are 100% recombinant and free of other nucleases. The product is well-suited to specialty applications such as gene therapy research as the risk of introducing pathogens is minimized.
Through extensive purification and refolding steps, Edna’s Ultra-RNase A has 3x the activity in Kunitz units per mg compared to competing products in the market extracted from bovine pancreas.
What's In The Box:
The product includes one vial of recombinant enzyme.
Highlights:
Cloned from bovine pancreas and recombinantly produced in E. coli and purified to >99% purity
Refolded in-vitro and purified to remove non-active forms
3x activity in Kunitz units/mg compared to competing non-recombinant products
Well suited for routine molecular biology applications as well as specialized work that requires bovine-free products
Product Source:
An E. coli strain that carries the RNase A gene from Bos Taurus.
Unit Definition: One unit of Ribonuclease A activity will cause a decrease of 100% per minute in the value of E0 – Ef at pH 5.0 at 25°C for the reaction Ribonucleic acid + water --> Oligonucleotides in the presence of RNase A, where E is the absorbance at 300 nm with path length of 1 cm.
Concentration: 1 mg/mL
Storage Temperature: -20 °C
Molecular Weight: 13.7 kDa
Standard Applications:
Plasmid and genomic DNA preparation
Removal of RNA from recombinant protein preparations
In preparing DNA for real time PCR
In DNA preparations for sequencing
In addition, Edna Ultra-RNase A products have a unique range of advantages:
Rigorously tested against nonspecific nuclease and protease activity
Second-step purification post-refolding to ensure that only maximally active refolded protein is present in the final formulation
Highly active
Useful for specialty applications requiring bovine-free products such as gene therapy research
View Product Data Sheet with Usage Protocol for Ultra-RNase A, Recombinant
View Latest Certificate of Analysis for Ultra-RNase A, Recombinant
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